Наукові праці. Кафедра гістології, цитології та ембріології
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Item Age-related changes in the cerebral hemispheres of male and female brains: a morphometric study using MRI brain scans(Bulletin of Medical and Biological Research, 2024) Maryenko, N.; Мар'єнко, Наталія ІванівнаUnderstanding the differences in brain ageing between males and females and the varying sensitivity of morphometric parameters to ageing are crucial for developing algorithms and protocols for objective and quantitative brain morphology evaluation in clinical practice. The study revealed more pronounced changes in absolute cross-sectional area values corresponding to overall brain tissue with ageing in males. However, no significant sex difference was observed in the age dynamics of relative values. The ratio of two cross-sectional brain areas, considering sulcal content and excluding it, has been identified as the most sensitive parameter to age-related changes in both male and female brains. This ratio could serve as an additional morphometric parameter for diagnostic purposes in examining cerebral structure.Item Anatomical variability of the alveolar process of the maxilla based on multislice computed tomography data(2024) Shiyan, D.; Kysylenko, K.; Trach, O.; Трач, Ольга Олександрівна; Yurevych, N.; Lupyr, M.; Alekseeva, V.Modern research methods, widely implemented in routine medical practice, open new horizons for the study of anatomical structures. The maxilla is one of the regions of the human skull that shows significant variability with age and gender. This is due to the peculiarities of tooth eruption and age-related changes in the periodontium and adjacent structures, which undoubtedly affect the structure of the alveolar process, sometimes drastically altering it over time. Aim: The aim of our study was to determine the anatomical variability of the alveolar process of the maxilla based on multislice computed tomography data. Materials and Methods: The research was conducted based on the results of 400 spiral computed tomography scans of males and females aged 18 to 95 years with detection of the anatomical variability of the alveolar process.Item Anatomical variants of the structure of the coronary arteries of the heart(ТДМУ, 2013-10) Масловський, Сергій Юрійович; Масловский, Сергей Юрьевич; Maslovsky, Sergey; Дуденко, Владимир Григорьевич; Дуденко, Володимир Григорович; Dudenko, Volodymyr; Бондаренко, Д.А.; Бондаренко, Д.А.; Bondarenko, D.Item Anatomo-morphometric features of human renal pyramids(ТДМУ, 2013-10) Масловський, Сергій Юрійович; Масловский, Сергей Юрьевич; Maslovsky, Sergey; Дуденко, Володимир Григорович; Дуденко, Владимир Григорьевич; Dudenko, Volodymyr; Шуба, Д.Г.; Шуба, Д.Г.; Shuba, D.Item Applications of fractal analysis techniques in magnetic resonance imaging and computed tomography for stroke diagnosis and stroke-related brain damage: a narrative review(Advanced Technology in Neuroscience, 2024) Maryenko, Natalia; Мар'єнко, Наталія ІванівнаThis review provides an overview of the principles, characteristics, and main applications of the fractal analysis technique, focusing on its applications and perspectives in stroke diagnosis based on neuroimaging data. In stroke research, the fractal analysis technique has been used to characterize brain tissue, pathological foci, and the vascular network, providing critical diagnostic and prognostic information. Researchers have applied the fractal analysis technique to brain lesions resulting from ischemic strokes to conduct geometric analyses of lesion shapes, indicating its diagnostic and prognostic values. Overall, the fractal analysis technique in brain magnetic resonance imaging and computed tomography is an informative and sensitive imaging analysis method that, with further development, can significantly improve stroke diagnosis and prognosis on the basis of neuroimaging data.Item Arbor vitae cerebelli: fractal properties and their quantitative assessment by novel “contour scaling” fractal analysis method (an anatomical study)(Translational Research in Anatomy, 2024) Maryenko, Natalia; Мар'єнко, Наталія Іванівна; Stepanenko, Oleksandr; Степаненко, Олександр ЮрійовичArbor vitae cerebelli (tree-like branching white matter of the cerebellum) has a complex spatial configuration that is challenging to assess using conventional morphometric methods. This study proposes a fractal approach to describe and quantify the anatomy of Arbor vitae cerebelli. For this purpose, a new “contour scaling” method for fractal analysis of cerebellar white matter was developed. The fractal (Hausdorff) dimension, determined using the novel “contour scaling” method, quantitatively assesses the degree of branching of the cerebellar white matter. An increase in the absolute size of the cerebellum leads to a higher degree of branching of its white matter and an increase in the number of its constitutive components – white matter branches and folia.Item Atrophic age-related changes in cerebral hemispheres: Euclidean geometry based morphometry of MRI brain scans(2023) Мар'єнко, Наталія Іванівна; Maryenko, Nataliia; Степаненко, Олександр Юрійович; Stepanenko, OleksandrThe aim of the present study was to conduct a comprehensive morphometric analysis of two-dimensional MRI brain images and determine the simple morphometric parameters of the cerebral hemispheres that best characterize quantitatively brain atrophic changes in normal aging. This study analyzed MRI brain scans from 100 apparently healthy individuals (44 males and 56 females) aged 18 to 86 years (mean age 41.72±1.58 years). For each brain investigation, five tomographic sections were selected, including four coronal and one axial. The perimeter, area values, and their derivative indices were determined. The study has shown that the parameter most sensitive to aging changes was the ratio of two area values: the area corresponding specifically to cerebral tissue and the area that captures the cerebral tissue and the sulcal content. The results of the present study can be used in clinical practice for the quantitative assessment of age-related atrophic changes in cerebral hemispheres.Item Auto nosodes from apoptotic cells of the buccal epithelium(2016) Карамишев, Василь Дмитрович; Карамышев, Василий Дмитриевич; Karamyshev, Vasiliy; Панасенко, В'ячеслав Олексійович; Панасенко, Вячеслав Алексеевич; Panasenko, Viacheslav; Клочко, Наталія Іванівна; Клочко, Наталия Ивановна; Klochko, NataliyaItem Characterization of white matter branching in human cerebella: quantitative morphological assessment and fractal analysis of skeletonized MR images(2021) Maryenko, Nataliia; Stepanenko, OleksandrIntroduction: The aim of the present study was to investigate branching characteristics of the human cerebellar white matter by the means of findings obtained from the quantitative morphological assessment and fractal analysis of the skeletonized MR images of the human cerebellum. Methods: Thirty individuals with no apparent brain pathology (15 males and 15 females, ranging from 18 - 30 years of age) participated in this study. Their normal T2-weighted MR images of the cerebellar vermis (midsagittal plane) were examined. The skeletonizing procedure and subsequent quantitative morphological assessment of the acquired skeletonized MR images were performed. The following parameters were determined: the number of branches, the number of junctions, the amount of end-point voxels, junction voxels and slab voxels, the average and maximum branch lengths, the longest-shortest patch length, and the number of triple and quadruple points. Additionally, the individual branches of the obtained digital skeletons of the cerebellar white matter were examined and the following parameters were assessed: branch length variability, Euclidean distance, and branch length/Euclidean distance ratio. A fractal analysis was performed using the box counting method prior to and after the MR image skeletonizing procedure. The values of the fractal dimensions (FD) of both skeletonized and non-skeletonized MR images were calculated.Results:It was established that the cerebella, which had the maximum values of the FD, possessed a large number of small branches approximately equal in length and which were connected by numerous junctions, forming numerous endpoints. Those cerebella, which had higher values of the average branch length and greater branch length variability, showed lower values of the FD. The key characteristics of the digital skeleton that determined the values of the FD of the cerebellum and its skeletonized MR images were the number of branches and the number of junctions that had the strongest correlational relationships with the FD of the skeletonized MR images. We submitted a proposition to consider the number of branches and amount of junctions as a diagnostic criterion in the determination of normal values of the FD. Conclusions: The obtained data can be used as diagnostic criteria in assessment of the orphofunctional state of the cerebellum using magnetic resonance imaging (MRI) technique.Item Comparative analysis of fractal dimensions of human cerebellum: impact of image preprocessing and fractal analysis methods(2022) Maryenko, Nataliia; Stepanenko, OleksandrThe aim: To compare the values of the fractal dimensions of human cerebellum obtained using different algorithms of image preprocessing and different methods of fractal analysis. Materials and methods: The study involved 120 people without structural changes in the brain (age 18-86 years, 55 men and 65 women). T1- and T2-weighted MR brain images were studied. Fractal analysis was performed using box counting and pixel dilatation methods. Fractal dimensions of cerebellar tissue as a whole, cerebellar cortex and its individual layers, cerebellar white matter were measured and compared to each other and to fractal dimension of cerebellar white matter determined in cadaveric cerebella. Results: It was no significant difference between fractal dimension values of cerebellar tissue as a whole measured on T1 and T2 weighted magnetic resonance images of cerebellum, and fractal dimension values measured on the same images using different methods of fractal analysis – pixel dilatation and box counting. T2 weighted images are preferable for fractal analysis of different components of cerebellar tissue. Segmentation according to pixel luminance is the preferable image preprocessing method for fractal analysis of cerebellar cortex as a whole, individual cortical layers and cerebellar tissue as a whole; skeletonizing of cerebellar magnetic resonance images is the preferable method of the image preprocessing for fractal analysis of cerebellar white matter. Conclusions: The algorithm of image preprocessing, magnetic resonance imaging sequence and method of fractal analysis should be chosen according to aim of quantitative study of cerebellar magnetic resonance images and features of the studied structure of cerebellum.Item Comparative clinical and morphological analysis of the periodontitis of different genesis(Видавництво ХНМУ, 2015) Деєва, Тетяна Володимирівна; Деева, Татьяна Владимировна; Deieva, Tetiana; Babay, Oksana; Merkulova, Yu.Модель пародонтита при введении бактериального эндотоксина позволяет направленно вызывать у животных комплекс воспалительно-дистрофических изменений пародонта, типичных для пародонтопатий у человека и патогномоничных пародонтиту человека, обосновывает целесообраз-ность использования данной модели при изучении пародонтопротекторной активности новых лекарственных средств.Item Cortex and white matter of the cerebral hemispheres: anatomical correlations and age-related changes measured with fractal analysis(2024-05) Maryenko, Nataliia; Мар'єнко, Наталія Іванівна; Stepanenko, Oleksandr; Степаненко, Олександр ЮрійовичThe aim of the present study was to determine the fractal dimension (FD) values of the cortex and white matter of the cerebral hemispheres using fractal analysis of two-dimensional magnetic resonance images, explore the anatomical correlations of the cortex and white matter FD, and study age-associated changes in the cortex and white matter. In this study, a negative correlation was found between the cortex and white matter FD values, influenced by anatomical factors such as the degree of gyrification. While the cortex FD values significantly decreased with age, age-related changes in the white matter FD values were relatively weak.Publication Definition of the range of variability of the linear parameters of the human skull depending on gender and craniotype in the age aspect(2024) Melnyk, Bogdan; Мельник, Богдан Ігорович; Boiagina, Olga; Боягіна, Ольга ДмитрівнаThe question of studying sexual dimorphism, age-related changes and aging of tissues, including bone tissue, is still relevant today. The rapid development of maxillofacial surgery and orthodontics requires an in-depth study of the features of skull formations depending on gender and craniotype. The purpose of our study was to determine the patterns of sexual dimorphism of craniometric indicators of the human skull depending on age. The material of the study was 125 CT scans of the head of men and women aged 25 to 85 without pathology of the bones of the skull, performed using a Neusoft NeuViz 16 Essence 16-Slice CT Scanner System. Visual analysis and craniometric measurements were performed using the Horos ver.4.0.1 program included in the computer tomography software and the Vidar Dicom Viewer ver. 3.3.1.9. It was established that the values of the linear dimensions of the skull, such as length, width and height, depend on the craniotype and gender of the person. The range of variability of the main indices of the skull was also determined. The most common combinations of skull types have been established.Publication Determination of the range of variability of the human orbital opening height and width according to computer tomography data(Дніпровський державний медичний університет, 2024) Melnyk, B.; Boiagina, O.; Боягіна, Ольга Дмитрівна; Panasenko, V.; Панасенко, В'ячеслав ОлексійовичUsage of computerized tomography scanning markedly expands now the ability for studying the features of the human skull structures. Morphometric studies play an important role in the identification of a person during a forensic medical examination, in determining the features of certain anatomical structures, including the orbit. Acquired during research findings may be useful in clinical medical practice when preparing for orbital reconstruction surgery after injuries or congenital malformations. Aim of study. Establishing the range of individual anatomical variability of the height and width of the human orbital opening depending on the craniotype and gender.Item Development of the related anemic syndrome in various types of endocrine pathology(2020) Piriatinska, Nataliia; Пірятінська, Наталія Євгенівна; Пирятинская, Наталья Евгеньевна; Vdovichenko, V.; Bakuridze-Manina, V.; Nekhanevych, O.; Yurevych, N.; Trach, Olga; Трач, Ольга Олександрівна; Трач, Ольга Александровна; Saprichova, L.; Zolotko, KyryloAnemia is a public health problem in almost all countries in the world, negatively affects human health, affects all sectors of the population and creates serious negative socio-economic consequences. The fact of anemia is the basis for stating a pathological condition that requires a nosological diagnosis with the identification of the causes of anemia. The article presents a review of modern literature, covering the impact of endocrine pathology on the development of various types of anemia in humans. The importance of biologically active substances in normal erythropoiesis is described, the role of erythropoietin and the action of hormones of the endocrine glands on its formation are considered. Endocrine pathologies such as hypo- and hyperthyroidism, hypo- and hyperparathyroidism, diabetes mellitus, insufficiency of the pituitary, hypothalamus, sex glands, adrenal glands and features of the development of anemic conditions are discussed. An analysis of the literature showed that an increase or decrease in the function of individual endocrine glands leads to a disruption in the formation of erythropoietin, impaired absorption of iron, folic acid and vitamin B12 and, as a result, ineffective erythropoiesis. Anemia negatively affects the course of the underlying disease, significantly worsening its prognosis, and its treatment is more often effective only in conjunction with the adjustment of hormonal status. Despite significant progress in the study of the pathogenesis of anemia, the emergence of new scientific data on the issues and mechanisms of regulation of erythropoiesis, classification options for anemia are still being discussed, the criteria for diagnosing a number of anemias and its combination with chronic diseases of endocrine and non-endocrine nature are being reviewed.Item Effects of anthropometric factors on human cerebellum weight and its developmental dynamics(2015) Степаненко, Олександр Юрійович; Степаненко, Александр Юрьевич; Stepanenko, OleksandrThe aim of the present work was to study the relationship between cerebellum weight and its evelopmental dynamics on the one hand and body length and type of physique on the other. Studies involved 295 corpses of both genders (173 men and 122 women) dying at age 20–99 years. Body length was measured, along with the cross-sectional diameter of the thoracic cage and cerebellum weight. Somatotypes were identifi ed using the Rice-Eysenck index. Human cerebellum weight from was found to vary over the range 103–197 g (mean 144 ± 1.0 g) and was signifi cantly greater in men than women (150.5 ± 1.3 g and 133.9 ± 1.2 g, p < 0.001). Age had a greater infl uence on cerebellar weight in men than in women (R = –0.46 and –0.43, respectively). In men, the period of relatively stable cerebellar weight lasted to about 50 years of age, which was followed by a period of decreasing cerebellar weight. In women, the stable period lasted to about 70 years. Cerebellar weight was related to body length (R = 0.35 for men and R = 0.36 for women). The relationship between cerebellum weight and body length in men (1.0 g/cm) was greater than that in women (0.5 g/cm): the differences in cerebellum weights in men and women increased with increases in body length. Differences in cerebellum weight in people with different types of physique were minor.Publication Establishing the range of variability of the longitudinal dimensions of the lower jaw in women with different craniotypes(International Scientific Unity, 2024) Melnyk, Bohdan; Boiagina, Olga; Боягіна, Ольга Дмитрівна; Samosudova, Liudmyla; Самосудова, Людмила ВікторівнаIn recent years, the areas of surgery related to the correction and plasticity of the bones of the facial skull have been developing rapidly, which in turn requires indepth knowledge of the individual anatomical variability of the structures of this area. Therefore, the further study of the peculiarities of the dimensions and relationships of the skull structures remains one of the most important problems of modern craniology. The purpose of the research is to determine the range of variability of the longitudinal dimensions of the lower jaw of women depending on the type of facial skull structure.Publication Establishing the range of variability of the width and height of the human visceral skull depending on the craniotype and gender(Дніпровський державний медичний університет, 2024) Melnyk, B.; Boiagina, O.; Боягіна, Ольга ДмитрівнаThe range of variability of the width and height of the facial part of the human skull, depending on the extreme forms of the structure, has been established. The results were obtained, according to which the linear dimensions of the skull that we studied are directly dependent on the craniotype: the largest indicators of the width of the facial part of the skull are characteristic of euryprozops, and the smallest – leptoprozops. It was determined that the most significant difference between the above-mentioned indicators, namely their increase in men compared to women, is characteristic of advanced age and old age. There were no statistically significant differences in the width and height of the facial skull in representatives of the same sex, but different age groups. Diagrams of the ratio of various types of facial skull structure according to the main facial index were created and the most common combinations of them with types of skull structure according to the cranial index were established.Item Evaluation of cerebellar aging in mri images: fractal analysis compared to euclidean geometry-based morphometry(Meta-Radiology, 2024) Maryenko, N.; Мар'єнко, Наталія Іванівна; Stepanenko, O.; Степаненко, Олександр ЮрійовичThis study aimed to identify age-related changes in the fractal dimensions of the cerebellum and compare the sensitivity of fractal analysis and conventional Euclidean geometry-based morphometry to cerebellar aging. The normal aging of the cerebellum involves not only absolute size alterations but also changes in the texture and spatial configuration of cerebellar tissue components, which can be quantitatively and objectively assessed by fractal analysis.Item The experience of Zoom employment for practical seminars in histology, cytology and embriology(ХНМУ, 2021) Stepanenko, Oleksandr; Hubenko, Irina; Novikova, K.