Кафедра гістології, цитології та ембріології
Permanent URI for this communityhttps://repo.knmu.edu.ua/handle/123456789/146
Browse
42 results
Search Results
Item Quantitative assessment of structural complexity in human cerebellum through analysis of skeletonized mr images: anatomical correlations, sex differences, and agerelated changes(Acta Morphologica et Anthropologica, 2024) Maryenko, Nataliia; Мар'єнко, Наталія Іванівна; Stepanenko, Oleksandr; Степаненко, Олександр ЮрійовичThe present study provides a quantitative assessment of the human cerebellum structural complexity using analysis of skeletonized images. Magnetic resonance images from 100 apparently healthy individuals (aged 18-86 years) were examined. Following segmentation, the images were skeletonized, and quantitative analysis of the digital skeletons was conducted. The following parameters were determined: number of branches and their junctions, end-point, slab and junction pixels, average and maximum branch length, triple and quadruple points. Sex differences were assessed. Correlation analysis included determining the relationships between the studied parameters and age, morphometric parameters derived from Euclidean and fractal geometries, as well as the same parameters of the digital skeletons identified in the cerebral hemispheres. In conclusion, quantitative analysis of digital cerebellar skeletons offers advantages for assessing the structural complexity of the cerebellum. This method and the results of the present study can be applied in diagnosing cerebellar malformations and distinguishing between malformations and atrophic alterations.Publication Individual variability of human cerebellar arteries and their perfusion territories(Morphologia, 2024) Калініченко, Михайло Олександрович; Kalinichenko, Mykhailo; Степаненко, Олександр Юрійович; Stepanenko, OleksandrBackground. Three paired arteries provide the blood supply of the cerebellum: the superior cerebellar artery, anterior inferior cerebellar artery, and posterior inferior cerebellar artery. The origin of these arteries, the extent of their development and their duplication may serve as factors influencing variations in the vascular supply territories. The aim of this study was to determine the characteristics of individual variability of the human cerebellar arteries and their perfusion territory. Methods. The study was conducted on 100 samples. Each sample included cerebellum and an adjacent brainstem. They were obtained from adult human cadavers (67 male and 33 female) who died of causes unrelated to brain pathology at the age between 20 and 92. To analyze the variability of perfusion territories of the cerebellar arteries, a method involving sectorial division of the superior and inferior surfaces of the cerebellum was proposed. Results and conclusion. In 95 samples, the SCA arose from the basilar artery on both sides as a single vessel. In two samples, it arose as a duplicate trunk from the basilar artery bilaterally. We also found unilateral duplication of the left SCA in three samples. The AICA arose from the lower third of the basilar artery in 69 samples on the right and in 77 on the left; from the middle third in 11 on the right and 11 on the left. It was presented as a common trunk with the PICA in 18 samples on the right and 10 on the left. The AICA was found duplicated in one sample bilaterally. In two samples it was absent on one side. The PICA most often arose from the vertebral artery (82 samples), rarely as a common trunk with AICA. It was duplicated in two samples on the left and absent in four samples on the right and four on the left. In cases of duplication of the PICA, its perfusion territory expands towards the central sectors of the inferior surface of the cerebellum. In the absence of the AICA, the PICA enlarges its perfusion territory, replacing it, and vice versa. Occasionally, the absent or poorly developed PICA is replaced by a PICA from the opposite hemisphere. There were not any cases of simultaneous absence of both AICA and PICA on one side. The probability of the extension of branches of the AICA onto specific sectors of the inferior surface of the cerebellum decreases from anterior to posterior and from the sides towards the center, while for the PICA there is an opposite trend. Additionally, this study describes three variations of the course of the arteries when both AICA and PICA originate as a common trunk from the basilar artery.Publication Shape and surface structure of the human cerebellum: variant anatomy(2023) Калініченко, Михайло Олександрович; Kalinichenko, Mykhailo; Степаненко, Олександр Юрійович; Stepanenko, OleksandrThe aim of the study was to determine the characteristics of individual variability in the shape and external structure of the human cerebellum. Cadaveric material (cerebella and adjacent brainstems of 100 people) was studied. We proposed a comprehensive method for evaluating the cerebellar shape in the morphometry of anatomical specimens, which involves measuring linear dimensions (width, length, and height) and calculating relative parameters, which describe cerebellar shape by the ratio of one dimension to the other two: relative width of the cerebellum (rW), relative length of the cerebellum (rL), and relative height of the cerebellum (rH). The magnitude of the relative parameter determines its contribution to the shape and surface structure of the cerebellum. The combination of relative parameters ultimately defines the characteristics of the shape and surface structure of the cerebellum, which were described in this study.Item Structure and individual anatomical variability of the lobules IV-V of the human cerebellar hemispheres(2017) Maryenko, Nataliia; Мар’єнко, Наталія Іванівна; Stepanenko, Oleksandr; Степаненко, Олександр ЮрійовичThe aim of the present study was to determine morphological variations in the lobules IV-V of the human cerebellar hemispheres. Material and methods. The research involved cerebella of 100 people of both sexes, who died of causes unrelated to brain pathology at the age of 20-95. The parasagittal sections of the cerebellum were investigated. Possible variations in the size, shape and peculiarities of branching of the white matter in the lobules IV-V of the cerebellar hemispheres were investigated. Results. Complex branched white matter is the basis of the IV-V lobules of human cerebellar hemispheres. The shape of the IV-V lobules is quite varied. Differences of the structure of these lobules depend on the characteristics of the branching of the white matter. The shape of the lobule depends on location of the section. Structure of the lobules is different on serial parasagittal sections. We described 2 variants of branching of the white matter of the hemispheric lobules IV-V at a distance 5 mm from midsagittal section (the 2nd variant is the most common) and 10 common variants of the shape of IV-V lobules (10 mm from midsagittal section); the third variant is the most common variant of the lobule IV; the first, second, and third variants are the most common shape variants of the lobule V. Conclusion. Described variants of the shape of the cerebellar lobules can be used as criteria standards of modern diagnostic imaging techniques for the diagnosis of various diseases of the CNS.Item Будова та індивідуальна анатомічна мінливість верхньої півмісяцевої часточки півкуль мозочка людини(2016) Мар’єнко, Наталія Іванівна; Марьенко, Наталия Ивановна; Maryenko, Nataliia; Степаненко, Олександр Юрійович; Степаненко, Александр Юрьевич; Stepanenko, OleksandrДосліджена будова та описані 10 варіантів розгалуження білої речовини верхньої півмісяцевої часточки півкуль мозочка людини. Найчастіше зустрічаються 1, 2, 3 та 5 варіанти форми, які можна вважати анатомічним стандартом. Отримані дані можуть бути використані в якості критеріїв норми для діагностичних методів нейровізуалізації. Исследовано строение и описаны 10 вариантов ветвления белого вещества верхней полулунной дольки полушарий мозжечка человека.Чаще всего встречаются 1, 2, 3 и 5 варианты формы, которые можно считать анатомическим стандартом. Полученные данные могут быть использованы в качестве критериев нормы для диагностических методов нейровизуализации. Cerebellum has the most complex spatial configuration which is associated with the organization of the arbor vitae («Tree of Life”) – tree-like branched white matter, which is structural basis of its cortex. Morphological changes of cerebellar lobules are found in many congenital and acquired diseases of the cerebellum. In recent years, thanks to modern imaging techniques (MRI, fMRI, CT, SPECT, PET) morphological changes of the hemispheric and vermal lobules that occur in these diseases can be detected in vivo, which is essential for early and accurate diagnosis. However, criteria of imaging diagnostic methods which are based on information about normal structure of the cerebellum, do not take into account the features of individual anatomical variability, gender and age characteristics. Objective – to investigate individual variability and features of variant anatomy of the superior semilunar lobule of the human cerebellar hemispheres. Research was conducted at the Kharkiv regional bureau of forensic medicine on 100 cerebellums of people of both sexes, who died of causes unrelated to brain pathology, 20-99 years old. During the forensic autopsy the cerebellum and brain stem were separated and fixed during one month in 10% formalin solution. Parasagittal sections of cerebellar hemispheres were investigated. Superior semilunar lobule of the human cerebellar hemispheres is formed by fifth branch of central white matter of the cerebellum. This lobule has pyramidal shape; the apex of pyramid is located in medial part of the hemisphere, the basis is located in lateral part of the hemisphere. The main trunk of white matter is the basis of this lobule. Main trunk divides into 2-3 branches which forms visible surface of the lobule. The shape of the superior semilunar lobule of the human cerebellar hemispheres is quite varied. Differences of the structure of this lobule depend on the characteristics of the branching of the white matter, the number and location of the secondary branches. We described 10 variants of the shape of the superior semilunar lobule of the human cerebellar hemispheres. The most common variants are 1st, 2nd, 3rd and 5th. That variants can be used as anatomical standard of the lobule. There are different number of little secondary branches on superior and inferior surfaces of the main trunk of white matter; 1, 2 or 3 branches can be found on superior surface, 1-4 branches can be found on inferior surface of the main trunk of white matter.Described variants of the shape of the cerebellar lobules can be used as criteria standards of modern diagnostic imaging techniques for the diagnosis of various diseases of the CNS.The data can be used as the basis for creation of the atlases of serial sections of the cerebellum, which are based on features of the individual anatomical variability of the cerebellum.Item Будова та індивідуальна анатомічна мінливість l-lll часточок півкуль мозочка людини(2016) Мар’єнко, Наталія Іванівна; Марьенко, Наталия Ивановна; Maryenko, Nataliia; Степаненко, Олександр Юрійович; Степаненко, Александр Юрьевич; Stepanenko, OleksandrМета дослідження – вивчити будову та встановити особливості інди- відуальної анатомічної мінливості І-ІІІ часточок півкуль мозочка людини. До- сліджена форма І-ІІІ часточок півкуль мозочка людини, описані 4 варіанти форми ІІ-ІІІ часточок, найбільш поширеним є 3-й варіант форми, який можна вважати анатомічним стандартом. Цель исследования – изучить строение и установить особенности индивидуальной анатомической изменчивости І-ІІІ долек полушарий мозжечка человека. Исследована форма І-ІІІ долек полушарий мозжечка человека, описаны 4 варианты формы ІІ-ІІІ долек, наиболее распространенным является 3-й вариант формы, который можно считать анатомическим стандартом. Morphological changes of cerebellar lobules are found in many congenital and acquired diseases of the cerebellum. In recent years, thanks to modern imaging techniques morphological changes of the hemispheric and vermal lobules can be detected in vivo. However, criteria of imaging diagnostic methods which are based on information about normal structure of the cerebellum, do not take into account the features of individual anatomical variability, gender and age characteristics. Objective - to investigate individual variability and features of variant anatomy of the I-III lobules of the human cerebellar hemispheres. Methods. Research was conducted at the Kharkiv regional bureau of forensic medicine on 100 cerebellums of people of both sexes 20-99 years old who died of causes unrelated to brain pathology. During the forensic autopsy the cerebellum and brain stem were separated and fixed during one month in 10% formalin solution. Parasagittal sections of cerebellar hemispheres were investigated. Results. The shape of the I-III lobules of the human cerebellar hemispheres is quite varied. Differences of the structure of these lobules depend on the characteristics of the branching of the white matter, the number of secondary branches and cerebellar folia. We described 4 variants of the shape of the II-III lobules of the human cerebellar hemispheres; 3rd variant is the most common. That variant can be used as anatomical standard of the lobules. Conclusion. Described variants of the shape of the cerebellar lobules can be used as criteria standards in current imaging techniques for the diagnostics of various diseases of the CNS.Item Фрактальный анализ как метод морфологического исследования мозжечка человека(Видавництво ВНМУ ім. М.І. Пирогова, 2017) Степаненко, Олександр Юрійович; Степаненко, Александр Юрьевич; Stepanenko, OleksandrItem Особенности формы мозжечка у больных с мальформацией Киари I типа(Видавництво ХНМУ, 2017) Степаненко, Олександр Юрійович; Степаненко, Александр Юрьевич; Stepanenko, Oleksandr; Меркулова, З.Л.The linear dimensions of the cerebellum and their ratio in standard and in patients with type I Chiari syndrome have been studied. A decrease in the absolute values of the height and an increase in the absolute values of the length of the cerebellum in patients with Chiari malformation have been established. It is shown, that the change in linear dimensions is reflected in the form of the cerebellum: in patients with Chiari malformation type I the cerebellum is relatively long and low, relatively broad and low, and is relatively long and narrow. The change in form is reflected in the position of the tonsils: a decrease in the relative width leads to a convergence of the tonsils along the midline, an increase in the relative length to their asymmetric arrangement, and a decrease in the relative height to their lowest position relative to the contour of the cerebellum. The change in the position of the tonsils due to the change in the shape of the cerebellum promotes their prolapse into the large occipital opening and the development of the Chiari syndrome type I.Item Особливості індивідуальної мінливості медіолатерального розміру часточок півкуль мозочка людини(Видавництво ХНМУ, 2017) Степаненко, Олександр Юрійович; Степаненко, Александр Юрьевич; Stepanenko, Oleksandr; Мар’єнко, Наталія Іванівна; Марьенко, Наталия Ивановна; Maryenko, NataliiaThe peculiarities of the individual variability of the mediolateral size of the hemispheric lobules of the human cerebellum, depending on the linear size of the cerebellum were investigated. Serial parasagittal sections of cerebellar hemispheres were studied. It was established, that the lobules I-X of the cerebellar hemispheres can end at a different distance from the median sagittal plane. The mediolateral size of the lobules of the cerebellar hemispheres is related to the width of the cerebellum: the farther from the median sagittal plane the lobules end, the greater the average width of the cerebellum. The length and height of the cerebellum are not related to the mediolateral size of the lobules.Item Закономерности индивидуальной изменчивости структуры поверхностной сосудистой сети мозжечка(ХНМУ, 2017) Степаненко, Олександр Юрійович; Степаненко, Александр Юрьевич; Stepanenko, OleksandrThe patterns of variability in the structure of the cerebellar superficial vascular network, its dependence on the mass and shape of the cerebellum are investigated. Morphometry was carried out by the method of fractal analysis with the calculation of the values of the fractal index. It is established that the structure of the superficial vascular network of envy from the mass – with increasing mass, the branching of the vasculature increases, which is manifested by an increase in the values of the fractal index. Fractal index of the superficial vascular network also increases with an increase in the relative length and height and a decrease in the relative width of the cerebellum.