Кафедра біологічної хімії
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Item Experimental Study of the Effect of GdYVO4:Eu3 Nanoparticles on the Metabolic and Histologic Features of Liver(2024) Gubina-Vakulik, G.; Nakonechna, О.; Gorbach, T.; Denisenko, S.; Balak, A.; Klochkov, V.; Yefimova, S.Item The role of pro-inflammatory cytokines in rats after implantation of polypropylene surgical mesh with a coating based on tantalum and its derivatives(2024) Nakonechna, O.; Kyslov, O.Since the beginning of the 21st century, polypropylene has become the main material used in hernia plastic surgery. More than 30 types of polypropylene surgical meshes are available in the world, but negative statistics regarding the development of the inflammatory process after implantation still remain disappointing. The development of the inflammatory process after implantation of polypropylene surgical meshes is observed in 30-40% of patients. Polypropylene nets, due to their synthetic nature and special physicochemical properties, can increase this inflammatory reaction. The body’s response to polypropylene materials can lead to encapsulation of the surgical mesh in fibrous tissue, a process designed to isolate the foreign material. This fibrotic reaction can cause discomfort, pain, and dysfunction of the mesh, causing subsequent deformation. In recent years, tantalum has been successfully used to produce biocompatible medical implants in surgery, orthopedics, and orthodontics. The aim of our study was to determine the content of pro-inflammatory cytokines, namely IL-1β and IL-6, in the blood plasma of experimental animals 28 days after implantation of polypropylene surgical meshes coated on the basis of tantalum and its derivatives, in particular tantalum oxide and nitride. The experimental group included 40 male rats of the WAG population. Experimental animals were divided randomly into six groups. With the help of surgical intervention, polypropylene surgical mesh «Omega II standard» (Ukrtehmed, Ukraine) measuring 15x15 mm with different types of coatings was implanted between the abdominal wall and the colon. 28 days after surgery, experimental animals were decapitated by cervical dislocation and blood was immediately collected to determine the content of IL-1β and IL-6 in blood plasma. The data obtained after the conducted studies indicate that coatings made of tantalum and tantalum oxide do not contribute to the emergence of an inflammatory process in comparison with the results of a group of experimental animals that were implanted with a surgical mesh without a coating. In the group of experimental animals, after implantation of a tantalum-based surgical mesh, the content of IL-1β and IL-6 was statistically higher by 14.6% and 21.6%, respectively, compared to the results in intact animals. In the group of experimental animals after implantation of a surgical mesh with a coating based on tantalum oxide, the content of IL-1β and IL-6 was statistically higher by 18.6% and 36.6%, respectively, compared to the results of the group of intact animals. At the same time, in the group of experimental animals after implantation of polypropylene surgical mesh without coating, the content of IL-1β and IL-6 was statistically higher by 72% and 81.6%, respectively, compared to the group of intact animals. As a result, the coating based on tantalum and tantalum oxide demonstrated an excellent anti-inflammatory effect and demonstrated excellent biocompatibility, which indicates to us the possibility of further improvement of surgical meshes with biocompatible coatings to achieve better results in surgical practice.Item Reactive oxygen species generation by blood leukocytes of rats after implantation of surgical mesh with tantalum-based coating(2024) Nakonechna, O.; Smachylo, R.; Dudin, S.; Kyslov, O.Introduction. Over the past 20 years, polypropylene has become the main material used in hernioplasty, and has proven itself as an excellent material for the restoration of the abdominal wall during hernia repair. Since polypropylene surgical meshes do not decompose well in the body, they can stimulate the development of an inflammatory process in the surrounding tissues, which subsequently causes adhesions. The development of a postoperative inflammatory process after implantation of polypropylene surgical meshes is observed in 30–40 % of patients. This affects the management of the postoperative period, increases the time of stay of patients in the hospital and their period of convalescence. These data force scientists to continue the search for the optimal surgical mesh, which would suit specialists not only from the side of the physical properties of the surgical mesh, but also from the side of its biocompatible and anti-inflammatory properties. Tantalum is successfully used to produce biocompatible medical implants in surgery, orthopedics and dentistry. In previous studies, we have repeatedly noted its excellent anti-inflammatory and antibacterial properties, indicating the possibility of its use as a coating for mesh implants. One of the typical responses to surgical intervention is the generation of reactive oxygen species by leukocyte neutrophils, which are signaling molecules that damage the endothelium of vessels and promote the migration of cells of the immune system to the center of inflammation. The aim of the study – to determine the generation of reactive oxygen species in leukocytes of rats of the control group and experimental rats with implantation of uncoated and tantalum-based surgical meshes. Research Methods. ROS generation was assessed in rat blood leukocytes using the dye 2,7-dichlorodihydrofluorescein diacetate (H2DCFDA) by flow cytometry 28 days after implantation of uncoated and tantalumcoated surgical meshes. Results and Discussion. Analyzing the obtained results, it was determined that the use of tantalum-based surgical meshes does not cause excessive generation of ROS by leukocytes, in contrast to the use of an implant without a coating. Implantation of uncoated surgical mesh caused excessive production of reactive oxygen species in blood leukocytes of rats, as evidenced by statistically significant differences in the mean fluorescence intensity of 2,7- dichlorodihydrofluorescein diacetate. Conclusions. The use of tantalum-based surgical meshes causes less generation of ROS in leukocytes compared to the use of uncoated surgical meshes, and does not provoke the development of adhesions and purulentseptic processes in the postoperative period, which is confirmed by a morphological study. This determines the possibility of their use in surgical practice to improve the durability and stability of use as biomedical implants and prevention of adhesion formation.