Наукові праці. Кафедра акушерства та гінекології № 2

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    Eryptosis contributes to gestational diabetes mellitus in maternal obesity
    (2024) Лазуренко, Вікторія Валентинівна; Железняков, Олександр Юрійович; Прокопюк Володимир Юрійович; Lazurenko, V.; Zhelezniakov, O.; Prokopiuk, V.
    Obesity is considered to be a risk factor for Gestational Diabetes Mellitus, which is characterised by hyperglycaemia in pregnant women. Recent studies have demonstrated that glycated erythrocytes are more prone to eryptosis, a unique regulated cell death observed only in mature erythrocytes.
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    Cesarian section in Ukraine and the United Kingdom: semilarities and differences
    (2024) Лазуренко, Виктория Валентиновна; Железняков, Олександр Юрійович; Алхімов Сергій Юрійович; Овчаренко Ольга Борисівна; Сафонов, Роман Анатолійович; Тертишнік, Деніс Юрійович; Lazurenko, V.; Zhelezniakov, O.; Alkhimov, S.; Ovcharenko, O.; Safonov, R.; Tertyshnyk, D.
    Negative statistics reveal that the increase in the frequency of CS is typical not only for the UK, but also for Ukraine, although in Ukraine the explanation for this situation is the increase in the frequency of obstetric and extragenital pathology during martial law, and in the UK it is the woman’s choice. Understanding the differences between countries in their approaches to CS will help improve the quality of medical care and optimize outcomes for both mother and newborn.
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    Eryptosis as a link between bacterial reproductive tract infection and anemia in pregnant women
    (2024) Кудін, Ілля Дмитрович; Лазуренко, Вікторія Валентинівна; Прокопюк, Володимир Юрійович; Железняков, Олександр Юрійович; Панасовський, Микола Леонідович; Kudin, I.; Lazurenko, V.; Prokopiuk, V.; Zhelezniakov, O.; Panasovskyi, M.
    Інфекції репродуктивного тракту бактеріального походження у вагітних жінок пов’язані з прискоренням процесів ериптозу, який може призводити до анемії у цих вагітних. Виключно при бактеріальній інфекції генітального тракту було показано, що запускається с кремблування із цього часу ериптоз в еритроцитах вагітних жінок.
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    Redox status and cell membrane alterations of circulating leukocytes and erythrocytes in abnormal uterine bleeding
    (2023-03-15) Alieksieieva, Olena; Harkavenko, Karyna; Posokhov, Evgeny; Prokopiuk, Volodymyr; Lazurenko, Viktoriia; Safonov, Roman
    Aim. To analyze the eryptosis degree, the state of cell membranes and redox status of circulating red blood cells and leukocytes in patients with abnormal uterine bleeding and its combination with hypothyroidism. Materials and methods. Patients, 74 women aged 18 to 49 years, were examined, which were divided into 3 groups: group I — women with abnormal uterine bleeding (AUB) (24 patients); group II — with AUB and thyroid pathology (30 patients, of whom 18 women had primary hypothyroidism and 2 — secondary hypothyroidism); group III — control group (20 healthy women, who had never had menstrual irregularities). Eryptosis of circulating erythrocytes was assessed by flow cytometry using annexin V staining and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) staining. Fluorescent probes O1O (2-(2¢-hydroxy-phenyl)-5-phe-nyl-1,3-oxazole) and PH7 (2-(2¢-hydroxy-phenyl)-phenanthro[9,10-d]-1,3-oxazole) were used to characterize changes in phospholipid bilayers of circulating erythrocytes and leukocytes. Lysed blood samples were stained with antibodies to CD45, 7-aminoactinomycin D and H2DCFDA to analyze the redox status of circulatingviable leukocytes.
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    Modern view on chronic respiratory dieseases in pregnant (review).
    (2022-09-30) Lazurenko, Viktoriia; Bilyi, Evgeny; Liashchenko, Olga; Ovcharenko, Olga; Borzenko, Iryna
    Disorders in the fetoplacental complex of pregnant women with chronic respiratory diseases (CRD) include a wide range of problems in modern obstetrics in medical, economic and social aspects. Respiratory diseases in the context of disorders of the fetoplacental complex (FPC) may be a comorbid process, a background to the abnormal pregnancy, or a premorbid condition that contributes to the development of placental dysfunction (PD) or even initiates it; morphophysiological changes characteristic of pregnancy also affect the state of the respiratory system, moderating the course of bronchoobstructive diseases. Respiratory diseases in women, the impact of its treatment and features of the course and medical support of pregnancy in these conditions affect the condition of both the woman and the fetus, and physical and neuropsychological development of the child in future.
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    Comparison of the effect of different mash implants on the reprodactive sestem in experimenral models of gynecologocal surgeries
    (2022) Сафонов, Роман Анатолійович; Прокопюк, Володимир Юрійович; Прокопюк, Олександра Вікторівна; Лазуренко, Вікторія Валентинівна; Тіщенко, Олександра Миколаївна; Овчаренко, Ольга Борисівна; Safonov, Roman; Prokopiuk, Volodymyr; Prokopiuk, O.; Lazurenko, Viktoriia; Tishchenko, O.; Ovcharenko, Olga
    Genital prolapse complicates the lives of many women after the age of 50. The use of mesh implants is a promising method of surgical correction of this disease. The study on mice compared the effect of the most common surgical meshes on the morphofunctional state of the reproductive system of animals, the postoperative period, and the completeness of morphological and functional recovery of the defects in the lower abdominal wall. It was found that thinner meshes cause less inflammatory reactions, faster recovery and less atrophic phenomena in the internal genitals. The recovery rate also depends on the material and structure of the mesh.
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    Modern approaches to the treatment of genital prolapse in obese women
    (2021) Safonov, Roman; Lazurenko, Viktoriia; Liashchenko, Olga; Afanasyev, I.; Garkavenko, K.; Chernyak, O.
    Introduction. The problem of female genital prolapse (GP) remains in the sportlight of gynecologists, because despite the variety of surgical methods, there are still recurrences of the disease, which are associated not only with the failure of the restored ligaments, fascia, muscles, damaged pelvic floor and perineum, but with the imperfection of the operation. The solution of this problem is especially important in the treatment of patients with extragenital pathology, in particular obesity. The purpose: to optimize the treatment of genital prolapse in obese patients by determining an individual approach to planning surgical treatment taking into account the degree of obesity and concomitant pathology. Materials and methods. We examined 65 patients of which 20 had genital prolapse and obesity (main group), 25 had genital prolapse and normal weight (comparison group), 20 women did not have gynecological diseases and extragenital pathology made up control group. To diagnose obesity and determine its degree we calculated body mass index (BMI). To determine the degree of GP its quantitative assessment was used (POP-Q; 1996). Surgical intervention included transvaginal extirpation of the uterus without appendages, anterior colporrhaphy, 325 colpoperineoraphy with levatoplasty, sacrospinal colpopexy. Transabdominal and laparoscopic colposacropexy in obese women were not used due to the presence of relative contraindications for laparoscopy (cardiovascular disease, respiratory pathology, adhesions, the condition after hernias’ surgery). Therefore, all operations on women with GP and obesity were performed transvaginally due to the inability to perform abdominal access. In comparison group transvaginal surgery was performed. All the groups under study were representative. Before the use of polypropylene mesh "Polymesh" to minimize purulent-septic complications associated with the use of synthetic prostheses aquadissection was performed with 0.9% saline with the addition of 1 g of ceftriaxone per 200 ml. After the operation, the women used suppositories with hyaluronic acid (revitax). Results. The results of surgical treatment have been analyzed and the following data were obtained: recurrences in the main and in the comparison group were 4% (2 women in whom operations were performed with the use of their own tissues without mesh prosthesis). Infectious complications, dyspareunia and pelvic pain were not observed. Conclusions. Surgical treatment of GP in obese women by using polypropylene mesh "Polymesh" for colposacropexy after transvaginal uterine extirpation increases the effectiveness of treatment and redduces the number of recurrences. Hydropreparation of the mesh with an antibacterial agent and postoperative use of hyaluronidase intravaginally helps to reduce purulent-septic complications of surgery and improves the patients’quality of life.
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    Ovarian function in patients who underwent the uterine arteries embolization
    (2021) Kalinovska, Olga; Lazurenko, Viktoriia; Cherepova, V.; Starkova, Iryna; Tishchenko, O.; Stryukov, D.
    The effect of uterine artery embolization on the ovarian function and blood flow in the ovaries was studied immediately after surgery and during the long-term postoperative period. Interruption of blood flow in the ovarian arteries immediately after UAE was noted. Restoration of blood flow was observed in women during 6-12 months of the postoperative period.
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    Isolation and Cryopreservation of Placental Cells: Search for Optimal Biotechniques in Experimental and Regenerative Medicine
    (2021) Prokopiuk, Volodymyr; Shevchenko, M.; Safonov, Roman; Prokopiuk, O.
    A high effi cacy of placental cells application necessitates their investigation. Preclinical studies require an improvement of the methods for obtaining, standardizing and storage of placental cells of experimental animals. Cells were isolated from rats and mice placentas by means of diff erent enzymatic methods and the one of explants. Cells were cryopreserved with DMSO in DMEM using two-stage freezing. The number, morphological, cultural, metabolic features of cells were studied after isolation and storage. The maximum number of viable cells from the placentas of mice and rats was found to be obtained using the explant method or trypsin with ETDA. Cell cultures from mice and rats placentas after the third passage had stable morphofunctional characteristics. Viability of warmed rat placental cells according to dye exclusion was (92.3 ± 1.6)%, according to the adhesive test this was (81.3 ± 5.8)%. For mice placental cells, these values were (86.7 ± 3.7)% and (79.2 ± 8.1)%, correspondingly. The research results enabled the determining of eff ective biotechniques for obtaining the cryopreserved placental cells of rats and mice to perform preclinical studies of their biological eff ect in models of allo- and autotransplantations.