Наукові праці. Кафедра судової медицини, медичного правознавства імені заслуженого професора М.С. Бокаріуса
Permanent URI for this collectionhttps://repo.knmu.edu.ua/handle/123456789/554
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Item Forensic medical assessment of morphological changesat different postmortem interval(2021) Grygorian, Edgar; Gubin, Mykola; Olkhovsky, Vasil; Shishkin, VolodymirPurpose: The postmortem interval (PMI) evaluation is one of priorities while performing aforensic medical examination of corpse. To date, there is lack of information on morphologicalpostmortem changes of some internal organs. Considering the persistent need to develop themethod for a precise assessment of PMI, postmortem changes in these potentially informativeorgans were evaluated. The aim of study was to analyze morphological postmortem changesin prostate and uterus. Materials and Methods: histological samples of 40 prostate and40 uterine tissues (n=80) from corpses of deceased aged 18–75 years. Only cases withknown time of death were included to study, the time of death was taken from policereports. Exclusion criteria were cases of violent death, death with massive blood loss, tumorsof studied internal organs and cases when diagnosis was not made by a forensic medicalexaminer. The PMI of studied cases ranged from 1 to 6 days. Histological slides were madewith a staining by hematoxylin and eosin, x200 magnification, using Olympus ÂÕ41 andOlympus ÂÕ46 microscopes, Olympus SC50 camera. Postmortem morphological changeswere evaluated by a calculation of blank spaces percentage in microscopical structuresusing a JS-based software. Relationship between PMI and morphological changes wascalculated by the Spearman's rank correlation. Results: the average percentage of blankspaces in uterine tissues was smaller than in prostate tissues (1.99 and 9.65, respectively).The slower growing of blank spaces was in uterus. In prostate samples, a notable increaseof blank spaces was observed between 48 and 72 hours after death. After this period, theincrease slowed down and then an increase was observed again between 120 and 144 hoursafter death. In uterine samples, a slight acceleration was observed between 72 and 120 hoursafter death and then slowing down between 120 and 144 hours after death. Blank spaces inevaluated histological slides were increasing directly proportional to the PMI, a statisticallysignificant interconnection was defined (p < 0.05). Conclusions: The morphological post-mortem changes in prostate and uterus were developing at certain time frames. Blank spacespercentage, in studied histological slides, were increasing directly proportional to the PMIincrease, a statistically significant interconnection was defined. Therefore, the results ofstudy show the possibility of the evaluation of a postmortem time interval by assessing suchmorphological changes in these organs, which could be used in forensic medical casesItem Postmortem Interval Assessment Criteria : A Retrospective Study(2020) Olkhovsky, Vasil; Kliuiev, Oleksandr; Grygorian, Edgar; Gubin, Mykola; Simakova-Yefremian, Ella; Khosha, VadymIntroduction, Materials and Methods : In this study, an analysis of forensic medical reports was conducted in order to define new criteria for postmortem interval (PMI) assessment, to improve the accuracy of its determination by forensic medical experts. In the study, 2972 forensic medical examinations of corpses were analyzed. The location of the corpse, Degree of fatness, Body length, Age and Histological changes of some internal organs were observed. Dynamometry method was used for Postmortem Interval (PMI) estimation. The results were calculated using Spearman rank correlation coefficient. Results : There was a significant moderate correlation of histological changes of some internal organs; weak correlation of corpse location, degree of fatness, body length; and significant very weak correlation of age (p <0.05).Item Relation of postmortem changes development and exact postmortem interval(2020) Grygorian, Edgar; Olkhovsky, Vasil; Gubin, MykolaPurpose: Precise postmortem interval evaluation is crucial in cases when violent types of death are suspected by a forensic medical examiner. There are different factors that could affect results of postmortem interval (PMI) evaluation by a forensic medical expert. The aim of this study was to investigate the relationship between the known postmortem time interval and the degree of particular postmortem changes development. Materials and Methods: A cross-sectional analysis of 116 forensic medical examinations of deceased persons (of them, 58 females and 58 males), in cases of non-violent death, was performed. The data about the time of death was obtained from police preliminary records provided to the examination - only the cases with known time of death were included in the study. Postmortem changes were evaluated by Total Body Score (TBS) [1] at equal time interval after death (48 hours ± 3 hours). Interconnection between postmortem changes degree and PMI was estimated using Spearman's rank correlation. Difference between sexes was evaluated using Mann-Whitney U test. Results: "Thickness of clothes" criterion reached the highest positive correlation coefficient, "ambient temperature" criterion had also a significant positive correlation. The rest of the studied criteria had very weak correlation with the development of postmortem changes. Conclusions: Several criteria had significant (p < 0.05), yet week, impact on the postmortem changes development. The other criteria were statistically insignificant.